A Comparative Study of ZiehlNeelsen Staining, Culture, and Line Probe Assay in Suspected Tuberculosis Patients Attending Tertiary Care Hospital in Northern India

Aishiya Ishrat, Sikander Chirag, Shashi Sudan Sharma


Background: Tuberculosis (TB) continues to be an emerging health problem in our country. Extrapulmonary TB accounts for 1520% of all cases of TB in addition to pulmonary TB. Diagnostic challenges in extrapulmonary TB remain to be addressed for both clinicians and microbiologists, along with Pulmonary TB. Materials and Methods: Samples from clinically suspected patients of TB received at the Department of Microbiology Government Medical College, Jammu. All samples were screened for acid-fast bacilli by 20% ZiehlNeelsen (ZN) stain, culture, and line probe assay. Results: A total (567) extrapulmonary samples were received during 1 and years. Out of which, 32 were found to be positive by ZN staining. Conclusion: Our results suggest that due attention and emphasis should be given on diagnosis and management of extrapulmonary TB and pulmonary TB, especially in resource-limited settings where persuasion is on smear microscopy finding.

Key words: Tuberculosis, Extra pulmonary tuberculosis, Pulmonary tuberculosis, Ziehl neelson stain, Acid fast bacilli

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